on a U-100 insulin syringe
| Concentration | — |
| Concentration | — |
| Volume per dose | — |
| Draw to | — |
| Doses per vial | — |
Figures update as you type. All values are for in-vitro research handling only — not for human or animal use.
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Browse the catalogThe reconstitution math, in plain terms
This peptide dosage and reconstitution calculator turns a lyophilized vial into an exact syringe draw. Reconstitution simply dissolves a lyophilized (freeze-dried) peptide into a liquid so it can be measured accurately. The concentration is the peptide mass divided by the volume of bacteriostatic water you add — nothing more. Once you know the concentration, the volume for any target dose follows directly, and a U-100 insulin syringe reads that volume in units (100 units = 1 mL).
Units to draw = dose ÷ concentration × 100
For example, a 10 mg vial reconstituted with 2 mL of water gives a concentration of 5 mg/mL (5000 mcg/mL). A 100 mcg target dose is 0.02 mL — which reads as 2 units on the syringe. For compound-specific guidance, see our guides on reconstituting BPC-157 and reconstituting retatrutide, or our overview of bacteriostatic water in Canada.
Reconstitution FAQ
It converts a lyophilized peptide vial into a precise draw on an insulin syringe. You enter the peptide mass in the vial (mg), the volume of bacteriostatic water you add (mL), and your target research dose. It returns the concentration, the volume per dose, the number of U-100 insulin units to draw, and how many doses the vial yields. All figures are for in-vitro research handling only.
There is no single correct volume — the amount of bacteriostatic water only sets the concentration. More water gives a lower concentration and a larger, easier-to-measure syringe draw; less water gives a higher concentration and a smaller draw. A common research choice is 1–3 mL per vial. Use the calculator above to see how a given volume maps to units on your syringe.
A standard U-100 insulin syringe is graduated in 100 units per 1 mL, so 1 unit equals 0.01 mL. A 0.3 mL syringe holds 30 units, a 0.5 mL syringe holds 50 units, and a 1.0 mL syringe holds 100 units. The calculator reports the draw in these units so you can read it directly off the barrel.
If the draw exceeds the barrel's capacity, the calculator shows a warning. Either add more bacteriostatic water to lower the concentration (which lowers the units per dose) or select a larger syringe. The concentration and doses-per-vial figures update instantly as you change the inputs.
For research handling, lyophilized (freeze-dried) peptides are stored at -20°C. Once reconstituted with bacteriostatic water, they are kept at 2–8°C and used within roughly 30 days. Bacteriostatic water itself can be stored at room temperature. Always follow the storage guidance on the product's documentation.
Yes. The reconstitution and dosing math is identical for any lyophilized peptide — BPC-157, retatrutide, tirzepatide, semaglutide, PT-141, GHK-Cu, and more. Use the product dropdown to auto-fill the vial mass from the Helix North catalogue, or type any mass in manually. The calculator returns the concentration and the exact insulin-syringe units to draw for your target research dose.